The point at which your line crosses zero on the y-axis is an approximation of the molar concentration of solutes inside the potato tuber cells this point identifies the molarity of a sucrose solution that has the same water potential as that of the potato tuber cells. According to clemson university, the molarity of a white potato is estimated between 024 and 031 when it is submerged in a sorbitol solution molarity stands for the number of moles of a solute required to dissolve in one liter of a solution the type of solution used will affect the molarity of. The potato cuboids placed into the sucrose solution with the molarities of 06, 08 and 10 lost weight showing that this is a hypertonic solution because it has a high concentration of water inside the potato cell due to the many solutes it contains. Ap biology lab: osmosis and potatoes what was the initial task 1 to determine the isotonic point of a sucrose solution and a potato 2 to determine an unknown concentration of a sucrose solution using a potato.
Writing an excellent potato osmosis lab report or osmosis in cells lab report requires a high level of knowledge in the field of study in potato cells lab report. Like cell membranes, dialysis tubing, which is usually made from regenerated cellulose, is semi-permeable, allowing smaller molecules across while blocking larger ones solutions can be put into the tubing by tying one end of the tubing, pouring the solution into the tubing and tying a knot to seal the other end of the tubing. Finding the concentration of a potato cell essay sample aim the aim is to find the concentration (mole/dm3 (m)) of solute in a potato cell by using the process of osmosis and different concentrations of sucrose solution. Below is a table of results of potatoes with the skin on: the starch concentrations were worked out using the published eu starch-density data in general the graph shows that heavier potatoes are denser and contain more starch.
In the osmotic concentration lab potatoes were used to see the affects of different concentrations of sucrose on the weight of the potatoes solanum tubersum more commonly known as the potato is the fourth most important food crop in the world. Osmosis is the diffusion of water across a semi-permeable membrane (yikes) from an area of high concentration of water, to an area of low concentration semi-permeable membrane : a layer that only certain things can go through. Osmosis is the movement of a solvent across a semi-permeable membrane from an area of lower solute concentration to an area of higher solute concentration the water (the solvent) can move across the membrane but the dissolved solutes (the sodium and chloride ions that form salt) cannot. When there is a weight change in the potato tuber from the sucrose solution the ones with a postive difference are hypotonic and the ones with a negative difference are hypertonic water moves from low sucrose molarity to high, meaning the potato will lose water making it lose weight.
Molar concentration of sucrose with water potential that is equal to the potato tissue water potential at this concentration there is no net gain or loss of ater from the tissue. Dns is a good method, pahbah is also an alternative here is the protocol, obtained from steve fry's 1988 book the growing plant cell wall: chemical and metabolic analysis. Considering the potato at the cellular level, explain the similarity of what happened to the potato cells in a concentrated sucrose solution with what happened to the elodea cells when salt water was added. The solute potential of the potato tuber cells is the same as the solute potential of a sucrose solution in which the potato cylinder did not change in mass the concentration of this sucrose solution (where ψsolution = ψcell) mass of the potato cylinder will occur can be determined using the trendline in figure 1.
At 04 m and higher, the potato cores actually lost mass, which indicates the outside concentration of water was less than inside the cores, so the concentration of solute inside the potato core was less than 04 m. I need results for the experiment of osmosis by only changing the concentration outside the potato but using the values volume of the total solution 20cm cubed the potato just using a normal cork borer cutting the pieces of. Lab 1c- potato/ water potential background: the concept of water potential is used to combine the differences in solute concentration and pressure to predict the direction in which water will diffuse through living plant tissues.
Finding the molarity of different sucrose solution using potatoes by maddy talbot the first 5 points did as expected and lost/ gained mass as they should have. Try to remember what happens when a cell is in hypothonic, isotonic and hypertonic solution which sucrose concentration fits in the hypothonic, isotonic and hypertonic solution when compared to the potato (hint: look a the percentage weight change between each sample. An investigation to determine the concentration of sugar inside potato cells - download as word doc (doc), pdf file (pdf), text file (txt) or read online scribd is the world's largest social reading and publishing site.
Therefore this solute potential of the potato tuber cell sap, can also be called the isotonic point of a potato cell a hypertonic solution contains a higher concentration of solutes, relative to the solute concentration of the solution within the cell itself, while a hypotonic solution contains a lower concentration of solutes, relative to the. Osmosis is the diffusion of fluid through a semipermeable membrane from a solution with a low solute concentration to a solution with a higher solute concentration until there is an equal concentration of fluid on both sides of the membrane. The cells inside the potato have had the water sucked out of them by the high concentration of salt in the water of the surrounding dish (for a more advanced understanding of cell structure, check out this course on biotech basics .
Lab: solute concentration of potatoes a solution is a solute (dissolved substances, including charged particles) suspended in a solvent (which in living systems is water. You could then chart the change in mass vs concentration of solution, work out when it crosses the 0, and that would be the concentration the same as that in the potato problems: does not account for concentration of other dissolved substances in cells. A mean value for the change in mass of potato cylinders at each concentration - measured in moles of sucrose per dm 3 of solution, or mol dm-3 - should be calculated.